Purification of antimicrobial factors from human cervical mucus
نویسندگان
چکیده
منابع مشابه
Purification of antimicrobial factors from human cervical mucus.
BACKGROUND The aim of this study was to separate bactericidal proteins from healthy female cervical mucus. METHODS Cervical mucus was collected and dissolved in 1% acetic acid. The antimicrobial activity of acid-soluble extracts was detected by gel overlay assay against Escherichia coli ATCC 43827. The extracts showed considerable amount of antibacterial activity with a clearly visible band. ...
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The antibacterial activity of human cervical mucus (CM) was examined on standardized microbial colonized agar plates (agar diffusion test). In parallel, the lysozyme content of CM was determined by means of a turbidimetric test system in aliquots of the same CM specimens. Suspensions of living lyophilized Micrococcus lysedeikticus were used as bacterial substrate. Testing was performed in a tot...
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Mucus glycoproteins (mucins) were extracted from human cervical pregnancy mucus by 6 M-guanidinium chloride in the presence of proteinase inhibitors. Purification was subsequently achieved by isopycnic density-gradient centrifugation in CsCl/ guanidinium chloride gradients. The purified macromolecules represented approx. 85% of the total and were devoid of nucleic acids and proteins, as judged ...
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ژورنال
عنوان ژورنال: Human Reproduction
سال: 2007
ISSN: 0268-1161,1460-2350
DOI: 10.1093/humrep/dem128